Which Platelet Index and Anticoagulation Method Should We Use? MPV or MPV/PC Ratio as an Index? EDTA or Citrate as Anticoagulant?

Abstract

Purpose: This study to compare the effectiveness of anticoagulation with ethylenediamine tetraacetic acid (EDTA) and citrate on MPV measurement.

Methods: EDTA and citrate-based anticoagulant blood samples from the same patients were read in the auto-analyzer at 0, 30, 60, and 120 minutes after sampling and the results were compared.

Results: A total of 54 patients, 29 of whom were women, over the age of 18 were included in the study. Baseline blood MPV values were found to be 0.524 fL greater in the EDTA-based group (p<0.001). When the difference between the time periods in the EDTA group was examined, it was observed that there was a significant increase in each deltaMPV value. When the difference between the time periods in the citrate-based group was examined, there was a significant difference at the 30th and 60th minutes (p<0.001), however the difference disappeared at the second hour (p>0.05). When the deltaMPV values of the EDTA and Citrate groups were compared, it was found that there was no difference at 30 and 60 minutes (p=0.531; p=0.566, respectively). In addition,it was found that there was no significant difference between deltaMPV/Platelet count ratios (deltaMPV/PC) in all time periods in the EDTA group (p>0.05) and there was a significant difference between all time periods in the citrate group (p<0.001).

Conclusions: Results in the first hour were similar in both anticoagulation groups. However, additional increases were observed in each half-hour period in both groups. When EDTA is used as an anticoagulant, MPV/PC ratio performs better than MPV.