Comparison of Transverse and Vertical Incision Techniques in Ventral Approach for Bilateral Ovariectomy in Rats
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Abstract
Objective: Ventral and dorsal surgical approaches are widely used for bilateral ovariectomy in animal experiments. The outcome of the surgery depends largely on the approach and incision used for conducting surgery. The present study compared the transverse and vertical incisions in the ventral approach with regard to the duration of the procedure, accessibility for the surgeon, number of sutures required, and time taken for wound healing and post-surgical mortality in rats. Design: A prospective study, conducted on 30 female wistar rats, at SGRRIM & HS, Dehradun for 6 months after approval from Institutional Animal Ethics Committee. Rats were randomly divided into 2 groups of 15 rats each. In Group A, rats underwent bilateral ovariectomy using transverse incision in ventral approach and in Group B, using vertical incision in ventral approach. Ketamine (80 mg/kg) and xylazine (10 mg/kg) were used intraperitoneally for anaesthesia. Comparison of surgical and post-surgical outcomes was done using Student T-test and Mann-Whitney U Test. The p-value < 0.05 was statistically significant. Results: The duration of surgical procedure in Group A was 15 ± 2.48 minutes and Group B was 29.47 ± 3.54 (p-value- 0.001), size of the incision in Group A was 1.35 ± 0.22 cm and Group B was 2.57 ± 0.40 (p-value- 0.001), number of sutures in Group A were 3.73 ± 0.80 and in Group B were 4.87 ± 0.92 (p-value- 0.003) and wound healing time in Group A was 2.87 ± 0.83 days and in Group B was 5.40 ± 0.83 days (p-value- 0.001). Conclusion: Study indicated that surgical and post-surgical outcomes by transverse incision for bilateral ovariectomy were significantly better than those by vertical incision. Further studies using a larger number of animals are needed to determine better incision in ventral approach.
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Copyright (c) 2025 Dr M. A Beg, Dr Shruti Malhotra, Dr Shalu Bawa, Dr Suman Bala, Dr Sangeeta Huidrom
This work is licensed under a Creative Commons Attribution 4.0 International License.
Creative Commons License All articles published in Annals of Medicine and Medical Sciences are licensed under a Creative Commons Attribution 4.0 International License.
Dr Shruti Malhotra, Assistant Professor, Department of Pharmacology, SGRRIM & HS, Dehradun-248001, India.
*Corresponding author: Dr Shruti Malhotra; Assistant Professor, Department of Pharmacology, SGRRIM & HS, Dehradun-248001, India.
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