Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) Test of Gambir Extract (Uncaria gambir Roxb.) and NaOCl Against Candida albicans as Root Canal Irrigation Material
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Abstract
Background: Root canal treatment failure that causes re-infection can occur due to various causes, one of which is by Candida albicans fungus in the root canal. Gambir extract is a natural ingredient that can be developed as a root canal irrigation solution, this plant has many benefits and contents, such as catechin and flavonoid compounds as antibacterial and antifungal. Purpose: This study aims to determine the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of gambir extract (Uncaria gambir Roxb.) and NaOCl on the growth of Candida albicans as a root canal irrigation material. Methods: The type of experimental laboratory research on the extraction of gambir plants (Uncaria gambir Roxb.) using the maceration method, fractionation to separate active compounds in gambir (Uncaria gambir Roxb.), then the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) test of gambir extract and 5% NaOCl using the broth microdilution method on 96 well plates observed with a microplate reader and the minimum bactericidal concentration (MBC) test using the spread method on agar calculated using a colony counter. Results: MIC of gambir extract (Uncaria gambir Roxb.) is 10% and MBC is 40% and MIC of NaOCl is 0,010% and MBC is 0,020%. Conclusion: Gambir extract (Uncaria gambir Roxb.) and NaOCl have effective minimum inhibitory concentration and minimum bactericidal concentration eagainst Candida albicans.
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